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S 2 is physiologically split because aortic valve closure normally precedes pulmonic valve closure. S 2 is caused by closure of the aortic and pulmonic valves at the beginning of isovolumetric ventricular relaxation. S 1 is normally slightly split (~0.04 sec) because mitral valve closure precedes tricuspid valve closure however, this very short time interval cannot normally be heard with a stethoscope so only a single sound is perceived. S 1 is caused by closure of the mitral and tricuspid valves at the beginning of isovolumetric ventricular contraction. The most fundamental heart sounds are the first and second sounds, usually abbreviated as S 1 and S 2.